N.B. The topic of the present Research Project was included on the International Research Database Industry Gateway’s Database(http://industrygateway.org/db/ ViewProject.do?projectID=5319&source=1&action=fetch) in the section Chemical Sciences/Analytical Chemistry/Other Analytical Chemistry/Large volume injection of sample diluents non-miscible with the mobile phase in RPLC: Principles and applications
The simplest and the most straightforward way for enhancing on the sensitivity of an analytical chromatographic method is the increase of the injected sample  volume. The golden rule of the thumb for large volume injection (LVI) in liquid chromatography (LC) is that the sample diluent should be entirely miscible to and  weaker than the mobile phase composition at the beginning of the separation. Effects of poorly controlled LVI conditions materialize in peak broadening and/or  symmetry distortions. The possibility of LVI of diluents non-miscible with the mobile phase was pioneered by our team, some theoretical aspects and applications  being already published. Considered as a natural continuation of the existing achievements, the objectives of the proposed project are focused on the following  topics relating to LVI of immiscible diluents in LC: a) fundamentals aspects and theory; b) a new insight about an adsorption theory in reversed phase separation  mechanism; c) relationships between analytes, diluents and separation mechanisms; d) the possibility of determining the hydrophobic character (log P) of  analytes based on LVI of immiscible diluents; e) experimental alternatives allowing LVI in immiscible diluents; f) possibilities for on-line automation of sample  preparation techniques to LVI in immiscible solvents; g) applications in bioanalysis, pharmaceuticals, environmental and food analysis. 
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